Which factors could lead to hemolysis of a blood sample?

Hemolysis refers to the rupture of red blood cells, which can release hemoglobin into the plasma, leading to potential complications in laboratory results. Several factors can contribute to hemolysis, making it essential to understand how different handling practices impact blood samples.

Using a syringe that is too small for the draw can create excessive pressure and turbulence as the blood is collected. This increased pressure can mechanically damage red blood cells, causing them to burst. Therefore, it's important to select an appropriate-sized syringe to minimize potential hemolysis during the collection process.

Mixing the blood sample vigorously is another common cause of hemolysis. When blood is shaken or mixed too forcefully, the mechanical action can disrupt the integrity of the red blood cells. Gentle mixing is recommended, especially after additives are introduced to anticoagulant tubes to ensure proper mixing without damaging the cells.

Considering both factors above, it becomes clear that they can work cumulatively to increase the likelihood of hemolysis. This understanding reinforces the importance of careful and appropriate handling techniques when collecting and processing blood samples.